← All IGCSE Biology 0610 Extended past papers
    Extended
    CAIE | IGCSE

    Biology (0610)

    0610/42 Oct/Nov 2025 Worked Answers (IGCSE Biology 0610 Extended)

    32 questions · 80 marks · 75 minutes

    Question papers and mark schemes are copyright Cambridge International. We do not reproduce them: the worked answers here are written by The Practice Book. Have the paper open alongside. Get the official paper from Cambridge

    Worked answers for 28 questions
    1. Step 1: Physical digestion breaks food into smaller pieces, for example by the teeth and the churning of the stomach. Step 2: It increases the surface area of the food but does not change the food molecules chemically. Step 3: Breaking molecules with enzymes is chemical digestion, absorption is the uptake into blood, and egestion is the removal of faeces.
      Method:
      Define physical digestion as a size reduction with no chemical change to the molecules.
      Examiner tips
      • Physical digestion = smaller pieces, larger surface area, no chemical change.
    2. Step 1: Bile emulsifies fat, breaking large drops of fat into many small droplets. Step 2: This greatly increases the surface area of the fat. Step 3: The larger surface area lets the enzyme lipase work faster, so bile aids physical digestion by preparing the fat for chemical digestion.
      Method:
      Link emulsification to increased surface area and faster enzyme action.
      Examiner tips
      • Emulsification = big fat drops into small droplets = more surface area for lipase.
    3. Step 1: Incisors are the chisel-shaped front teeth that cut and bite food. Step 2: Molars are the broad back teeth that grind and chew food. Step 3: Teeth do not absorb nutrients, make saliva or store food, so only the first option pairs each tooth with its correct function.
      Method:
      Name two teeth and give the cutting or grinding function that matches each.
      Examiner tips
      • Incisors and canines at the front cut and tear; premolars and molars at the back grind.
    4. Question 2(a)(i)

      2 marksMain features of the plant kingdom
      Step 1: Plants are multicellular organisms. Step 2: They are producers, making their own food by photosynthesis using chlorophyll in chloroplasts, and have cellulose cell walls. Step 3: Being single-celled, feeding on others, moving around or having chitin walls describe other kingdoms, not plants.
      Method:
      Pick the two statements that match the producer, multicellular nature of plants.
      Examiner tips
      • Plant features: multicellular, photosynthesise, chloroplasts, cellulose cell wall, non-motile.
    5. Question 2(a)(ii)

      2 marksNaming other kingdoms
      Step 1: The five kingdoms are animal, plant, fungus, protoctist and prokaryote. Step 2: The animal kingdom and the fungus kingdom are two of these, other than plants. Step 3: Leaves and flowers are plant parts, vertebrates and insects are groups within the animal kingdom, and viruses are not placed in a kingdom.
      Method:
      Name two of the recognised kingdoms that are not the plant kingdom.
      Examiner tips
      • Five kingdoms: animal, plant, fungus, protoctist, prokaryote.
    6. Step 1: In a monocotyledon stem the vascular bundles are scattered across the whole cross-section. Step 2: In a dicotyledon stem the vascular bundles are instead arranged in a ring towards the outer edge. Step 3: They are not scattered, not all in the centre, and they are certainly present, so only the ring arrangement is correct.
      Method:
      Contrast the scattered monocot arrangement with the ring arrangement of a dicot.
      Examiner tips
      • Dicot stem: vascular bundles in a ring; monocot stem: scattered.
    7. Step 1: In a dicotyledon root the xylem is found in the centre, often in a star shape. Step 2: The phloem lies between the arms of the xylem, around the central xylem. Step 3: Root hairs are extensions of the surface cells that project from the outer edge of the root, so only the first option places all three correctly.
      Method:
      Place xylem in the centre, phloem around it, and root hairs on the surface.
      Examiner tips
      • Dicot root: central xylem, surrounding phloem, surface root hairs.
    8. Question 2(d)

      2 marksFunctions of xylem
      Step 1: Xylem transports water and dissolved mineral ions from the roots up the plant. Step 2: The thick walls of xylem vessels also help to support the plant. Step 3: Transport of sucrose and amino acids is the job of phloem, and the other options describe processes xylem does not carry out.
      Method:
      Give the transport-of-water role and the support role of xylem.
      Examiner tips
      • Xylem: transports water and mineral ions, and supports the plant.
    9. Step 1: The girdled trees gave 1796 g of fruit compared with 958 g for the ungirdled trees, so girdling roughly doubled the fruit mass. Step 2: Phloem normally translocates sugars (sucrose) made by the leaves away from the branch towards other parts of the plant. Step 3: Removing the phloem traps these sugars in the branch above the cut, so more sugar is available for the growing fruit, giving a greater fruit mass.
      Method:
      State the comparison of masses, then explain using translocation of sugars in phloem.
      Examiner tips
      • Girdling removes phloem, trapping sugars above the cut so the fruit gets more.
    10. Step 1: Pectinase breaks down pectin in the cell walls of the fruit. Step 2: This releases more juice and makes the juice clearer, increasing the yield. Step 3: Protease digests protein, lipase digests fat and catalase breaks down hydrogen peroxide, so none of these is used to make fruit juice.
      Method:
      Choose the enzyme that digests pectin in fruit cell walls.
      Examiner tips
      • Pectinase breaks down pectin to release and clear fruit juice.
    11. Question 3(a)

      2 marksThe meaning of catalyst
      Step 1: A catalyst increases the rate of a chemical reaction. Step 2: It is not used up or chemically changed during the reaction, so it can be used again. Step 3: A catalyst speeds up rather than slows down a reaction, and it does not supply energy, so only the first option is correct.
      Method:
      Define a catalyst as a substance that speeds up a reaction without being changed.
      Examiner tips
      • Catalyst: speeds up a reaction, not used up or changed.
    12. Step 1: Iodine turns blue-black with starch, so a faster loss of the blue-black colour means the amylase has broken down the starch faster. Step 2: At 0 °C the particles have little kinetic energy, so there are few successful collisions between amylase and starch and the reaction is very slow. Step 3: As the temperature rises towards 40 °C the particles gain kinetic energy and collide more frequently and successfully, forming more enzyme-substrate complexes and breaking the starch down faster.
      Method:
      Explain the faster colour loss using increasing kinetic energy and successful collisions up to 40 °C.
      Examiner tips
      • Higher temperature (up to the optimum) = more kinetic energy = more successful collisions = faster reaction.
    13. Question 3(b)(ii)

      2 marksEnzyme specificity
      Step 1: Enzymes are specific, meaning each enzyme has an active site that fits only its own substrate. Step 2: Protease has an active site shaped for protein, not for starch, so starch cannot bind and no enzyme-substrate complex forms. Step 3: Because the starch is not broken down, it remains present and continues to turn the iodine blue-black at every temperature.
      Method:
      Use enzyme specificity to explain why protease leaves the starch undigested.
      Examiner tips
      • Specific enzymes only fit their own substrate; protease cannot digest starch.
    14. Step 1: Each enzyme has an optimum pH at which it works fastest. Step 2: Moving away from this pH slows the enzyme and can denature it, so pH affects enzyme action. Step 3: The colour of the container, the time of day and the mass of the test tube have no effect on the enzyme.
      Method:
      Name pH as a factor, besides temperature, that affects enzymes.
      Examiner tips
      • Besides temperature, pH affects enzyme activity.
    15. Question 4(a)(ii)

      1 marksConstructing a food chain
      Step 1: A food chain starts with a producer and the arrows point from food to feeder. Step 2: Grass (producer) is eaten by the grasshopper, which is eaten by the corsac fox, which is eaten by the steppe eagle. Step 3: This gives four trophic levels in the correct order, while the other chains have too few levels or the arrows the wrong way round.
      Method:
      Build the chain from grass up to the steppe eagle with four linked organisms.
      Examiner tips
      • Food chain: producer first, arrows from food to feeder, four levels here.
    16. Step 1: Energy is transferred along a food chain but a large amount is lost at each trophic level, for example as heat from respiration and in waste. Step 2: This means less energy is passed on to the next level. Step 3: With less energy available, less biomass can be supported, so there are fewer organisms at each higher trophic level.
      Method:
      Link the loss of energy at each level to fewer organisms being supported.
      Examiner tips
      • Energy lost at each level → less energy → fewer organisms higher up.
    17. Step 1: A small starting population contains only a little genetic variation. Step 2: Breeding among closely related individuals (inbreeding) increases the chance of harmful recessive alleles pairing up, raising the risk of inherited disease. Step 3: With little variation the species is also less able to adapt to environmental change, so the population is more vulnerable.
      Method:
      Explain the risk through reduced genetic variation, inbreeding and poorer adaptability.
      Examiner tips
      • Small population → little genetic variation → inbreeding → inherited disease, poor adaptability.
    18. Step 1: Protecting the habitat and passing laws against hunting keep the horses and their environment safe. Step 2: Monitoring the population and educating local people help conservationists respond to threats and gain support. Step 3: Removing habitat, allowing hunting, adding predators or causing pollution would all harm the population, so only the first option helps maintain it.
      Method:
      Select genuine conservation measures that protect the horses and their habitat.
      Examiner tips
      • Conservation methods: protect habitat, laws against hunting, monitoring, education, veterinary care.
    19. Step 1: The egg and sperm joining to form a zygote is fertilisation. Step 2: The zygote dividing repeatedly by mitosis to form an embryo is cell division. Step 3: The embryo settling into the uterus lining is implantation, so the order is fertilisation, mitosis, implantation.
      Method:
      Name each stage in the order the egg becomes a zygote, an embryo and then implants.
      Examiner tips
      • IVF order: fertilisation → mitosis (cell division) → implantation.
    20. Step 1: Keeping animals close together uses less land for a given output, so more land is free for other uses. Step 2: It gives a higher yield and a more consistent quality of product, often more cheaply. Step 3: These improve food security and profits, so the first option lists real advantages, while the others describe disadvantages or false claims.
      Method:
      Choose the three statements that genuinely benefit food production.
      Examiner tips
      • Intensive farming: less land, higher yield, cheaper and more consistent products.
    21. Step 1: In intensive systems many animals are kept close together. Step 2: This overcrowding lets bacterial infections spread quickly between animals. Step 3: Antibiotics are used to treat and help prevent these bacterial diseases, so the first option is correct, while antibiotics do not kill viruses or act as vitamins.
      Method:
      Link the close housing of animals to the spread of bacterial disease and the use of antibiotics.
      Examiner tips
      • Antibiotics treat and prevent bacterial disease that spreads in crowded conditions.
    22. Question 5(c)(ii)

      3 marksCalculating a percentage increase
      Step 1: The increase is 400 − 260 = 140 mg per kg. Step 2: Percentage increase = (increase ÷ original) × 100 = (140 ÷ 260) × 100 = 53.846…%. Step 3: Rounded to two significant figures this is 54%.
      Method:
      Calculate the increase, divide by the original 2010 value and convert to a percentage.
      Examiner tips
      • Percentage increase = (increase ÷ original value) × 100.
    23. Question 5(c)(iii)

      4 marksDevelopment of antibiotic resistance
      Step 1: A chance mutation in the DNA of some bacteria makes them resistant to the antibiotic. Step 2: When the antibiotic is used it kills the non-resistant bacteria, but the resistant ones survive and reproduce, passing on the resistance allele. Step 3: Over many generations the proportion of resistant bacteria increases, which is natural selection acting on the population.
      Method:
      Explain resistance as mutation followed by natural selection of surviving resistant bacteria.
      Examiner tips
      • Mutation → antibiotic kills non-resistant → resistant survive and reproduce → natural selection.
    24. Step 1: The breathing rate returns to its resting value 20 minutes after exercise, and the heart rate falls by 150 − 60 = 90 beats per minute between 20 and 40 minutes. Step 2: During exercise lactic acid builds up in the muscles, creating an oxygen debt, and it is carried in the blood to the liver. Step 3: Breathing stays high to supply oxygen, which is used in aerobic respiration to break down the lactic acid, so the first option is correct throughout.
      Method:
      Read the two graph values, then complete the oxygen-debt explanation with muscles, liver, oxygen and aerobic respiration.
      Examiner tips
      • Oxygen debt: lactic acid in muscles → carried to liver → broken down by aerobic respiration using oxygen.
    25. Question 6(b)(i)

      1 marksThe organ that produces urea
      Step 1: Excess amino acids are deaminated, removing their nitrogen-containing part. Step 2: This happens in the liver, where the nitrogen is converted into urea. Step 3: The kidney removes urea from the blood, the lungs deal with carbon dioxide, and the bladder stores urine, so the liver is the organ that produces urea.
      Method:
      Recall that urea is formed in the liver during deamination.
      Examiner tips
      • Urea is produced in the liver by deamination.
    26. Question 6(b)(ii)

      1 marksThe organ that excretes urea
      Step 1: Urea is carried in the blood from the liver to the kidneys. Step 2: The kidneys filter the blood and remove urea, which leaves the body in urine. Step 3: The liver makes urea but does not excrete it, and the pancreas and stomach are part of digestion, so the kidney is the excretory organ for urea.
      Method:
      Recall that the kidney filters urea from the blood and excretes it in urine.
      Examiner tips
      • The kidney excretes urea in the urine.
    27. Step 1: Anaerobic respiration in yeast breaks down glucose without oxygen. Step 2: The products are ethanol and carbon dioxide, giving C6H12O6 → 2C2H5OH + 2CO2. Step 3: The equation with oxygen is aerobic respiration, the lactic acid equation (C3H6O3) is anaerobic respiration in muscle, and the reversed equation is not respiration.
      Method:
      Choose the equation where glucose forms ethanol and carbon dioxide with no oxygen.
      Examiner tips
      • Anaerobic respiration in yeast: glucose → ethanol + carbon dioxide.
    28. Step 1: When bread dough is made, yeast respires anaerobically. Step 2: The carbon dioxide it releases forms bubbles that make the dough rise. Step 3: Cheese and butter are dairy products and salt is a mineral, so bread is the product made using yeast.
      Method:
      Pick the food whose production relies on carbon dioxide from yeast.
      Examiner tips
      • Yeast makes bread rise by releasing carbon dioxide.

    Sit this paper in the app

    Timed mock papers, instant marking and worked solutions for every question, free.

    Practise in the app